molecular characterization and antibiotic susceptibility pattern of caprine shiga toxin producing-escherichia coli (stec) isolates from india

نویسندگان

a. mahanti

i. samanta

s. bandyopadhyay

s. n. joardar

چکیده

the present study was conducted to detect the occurrence, serotype, genotype, phylogenetic relationship and antimicrobial resistance pattern of stec from healthy goats of west bengal, india. from the 125 faecal samples collected from healthy goats, 245 isolates were identified as escherichia coli. the e. coli harbouring any gene for shiga toxins (stx1/stx2) was detected in 36 (14.7%) of the 245 e. coli isolates. these stec strains belonged to 22 different serogroups (o2, o5, o20, o21, o22, o25, o41, o44, o45, o60, o71, o76, o84, o85, o87, o91, o103, o112, o113, o120, o156, and o158) and three were untypeable. the stx1 and stx2 was detected in 26 (72.2%) and 21 (58.3%) of shiga toxin producing-e. coli (stec) isolates, respectively. further, e. coli harbouring eaea only (enteropathogenic e. coli) and ehxa was detected in 22 (61.1%) and 28 (77.7%) isolates, respectively. whereas the saa was present in 8 (22.2%) e. coli isolates. the subtyping of the 26 e. coli strains possessing stx1 showed that 73.% (19/26) of these isolates were positive for stx1c subtype. of the 21 isolates with the stx2 gene, 42.8% (9/21) were positive for stx2c, and 38.1% (8/21) were positive for stx2d gene. the phylogenetic analysis of stec strains after rapd reveals eight major clusters. however, no serogroup specific cluster was observed. resistance was observed most frequently to erythromycin (80.5%), amikacin (52.7%), cephalothin (50%), kanamycin (41.6%), neomycin (36.1%) and gentamycin (36.1%) and less frequently to norfloxacin (2.7%), enrofloxacin (2.7%), and ciprofloxacin (2.7%). multidrug resistance was observed in eleven stec isolates.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Molecular characterization and antibiotic susceptibility pattern of caprine Shiga toxin producing-Escherichia coli (STEC) isolates from India

The present study was conducted to detect the occurrence, serotype, genotype, phylogenetic relationship and antimicrobial resistance pattern of STEC from healthy goats of West Bengal, India. From the 125 faecal samples collected from healthy goats, 245 isolates were identified as Escherichia coli. The E. coli harbouring any gene for Shiga toxins (stx1/stx2) was detected in 36 (14.7%) of the 245...

متن کامل

Molecular characterization and antibiotic susceptibility pattern of caprine Shiga toxin producing-Escherichia coli (STEC) isolates from India.

The present study was conducted to detect the occurrence, serotype, genotype, phylogenetic relationship and antimicrobial resistance pattern of STEC from healthy goats of West Bengal, India. From the 125 faecal samples collected from healthy goats, 245 isolates were identified as Escherichia coli. The E. coli harbouring any gene for Shiga toxins (stx 1/stx 2) was detected in 36 (14.7%) of the 2...

متن کامل

Molecular characterization of shiga like toxin-producing Escherichia coli (STEC) isolates from pigs oedema.

BACKGROUND & OBJECTIVE An oedema outbreak occurred in a Guwahati pig farm. Escherichia coli isolates from different necropsy samples collected from the dead piglets with oedema were characterized to confirm the virulence. METHODS Haemolytic E. coli isolates recovered from liver, lung and intestine of pigs with oedema were examined for presence of genes encoding pathogroups such as enteropatho...

متن کامل

Shiga toxin-producing Escherichia coli (STEC).

Shiga toxin-producing Escherichia coli (STEC) are important enteric pathogens worldwide, causing diarrhea with or without blood visibly present and hemolytic uremic syndrome. STEC are unique among diarrheogenic E coli in producing Shiga toxin type 1 and type 2, the virulence factors responsible for bloody diarrhea and hemolytic uremic syndrome. Cattle and other ruminants are the natural reservo...

متن کامل

Rapid, Multiplexed Characterization of Shiga Toxin-Producing Escherichia coli (STEC) Isolates Using Suspension Array Technology

Molecular methods have emerged as the most reliable techniques to detect and characterize pathogenic Escherichia coli. These molecular techniques include conventional single analyte and multiplex PCR, PCR followed by microarray detection, pulsed-field gel electrophoresis (PFGE), and whole genome sequencing. The choice of methods used depends upon the specific needs of the particular study. One ...

متن کامل

Detection of Shiga toxin-producing Escherichia coli (STEC) in faeces of healthy calves in Mashhad, Iran

The aim of this study was to identify virulent Shiga toxin-producing Escherichia coli (STEC) strains isolated from faecal samples of 100 clinically healthy calves. In the present study, a total of 100 Escherichia coli (E. coli) isolates from clinically healthy calves belonging to 6 different farms located in Khorasan Razavi province, Iran, were examined for presence of virulence genes character...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of veterinary research

ناشر: shiraz university

ISSN 1728-1997

دوره 16

شماره 1 2015

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023